Evaluation of the Benefits of Microfluidic-Assisted Preparation of Polymeric Nanoparticles for DNA Delivery

An effective delivery vehicle of genetic materials to their target site is the key a successful gene therapy. In many cases, nanoparticles are used as choice and efficiency relies heavily on physicochemical properties nanoparticles. Microfluidics, although being low throughput method, has been increasingly researched for preparation A range superior were claimed in literature microfluidic-prepared platforms, but no evidence direct comparison prepared by microfluidics conventional high method exists, leaving industry with little guidance how select large-scale nanoparticle manufacturing method. This study plasmid DNA-loaded PLGA-Eudragit model system critically compare microfluidics-assisted nanoprecipitation. The found be statistically significantly larger than ones conventionally showed higher DNA loading efficiency. Although both methods did not induce significant cytotoxicity, transfection was which good potential delivery. first time provides microfluidic methods. findings bring new insights into critical evaluation selection future • Polymeric (NP)-based therapy treat wide gene-associated diseases. Superior reported NPs microfluidics, challenges during scale-up. Eudragit-PLGA processes performed. results proved vectors. do translate transfection.